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Authors
Source
NIMHANS Journal, Vol 7, No 2 (1989), Pagination: 137-142
Abstract
A simple and sensitive method has been described for the demonstration of class specific antibody to Cysticerous cellulosae by passive haemagglutination (PHA) reaction through intact cells of Staphylococcus aureus Protein - A (SAPA). SAPA binds rapidly to Fc portion of IgG with high affinity without affecting the immunological reactivity of bond immunoglobulin. Double aldehyde stabilized (DAS) sheep red blood cells sensitized with soluble antigens from porcine cyst sonicate were used in the test. This study was conducted on 142 cerebrospinal fluid (CSF) specimens. Of these, 5 cases were confirmed neurocysticercosis, 66 were clinically suspected and 71 controls. For comparative evaluation, all CSF specimens were examined by enzyme linked immunosorbent assay (ELISA). The results indicate that PHA and SAPA-AMHA tests are sensitive methods in demonstration and quantitation of anticysticercal antibodies. The results also suggest that reaction was mediated predominantly by IgG and in some cases by both IgG and IgM which finally has importance in the diagnosis of the disease.
Keywords
Neurocysticercosis, Staphylococcus Aureus, Haemagglutination Assay, Cerebrospinal Fluid, ELISA
